The Seasiders start the group stages with a trip to the Brick Community Stadium to take on Wigan Athletic, with home games against Crewe and Aston Villa U21s coming next month.
Several players remain absent due to injury, while Bailey Peacock-Farrell, Dion Charles (both Northern Ireland), and Karoy Anderson (Jamaica) are all on international duty.
Here’s our predicted XI and bench to take on Wigan:
1. Luke Southwood
Luke Southwood is set to make his first appearance for Blackpool in Bailey Peacock-Farrell's absence. Photo: Gareth Evans
2. Andy Lyons
Andy Lyons could be among those to pick up some minutes. Photo: Gareth Evans
4. Zac Ashworth
Zac Ashworth was among those who struggled in Saturday's 4-2 defeat to Plymouth Argyle, but could slot into the back three from the start this evening. Photo: Gareth Evans
5. Josh Bowler
Josh Bowler has been one of Blackpool's brighter players so far this season. Photo: Gareth Evans
6. Jordan Brown
Jordan Brown has been building up his fitness over the last couple of weeks after suffering a seizure on the opening day of the season. Photo: Gareth Evans