The Seasiders start the group stages with a trip to the Brick Community Stadium to take on Wigan Athletic, with home games against Crewe and Aston Villa U21s coming next month.
Several players remain absent due to injury, while Bailey Peacock-Farrell, Dion Charles (both Northern Ireland), and Karoy Anderson (Jamaica) are all on international duty.
Here’s our predicted XI and bench to take on Wigan:
13. SUB: Jordan Williams
Jordan Williams could drop out of the starting XI to give opportunities to others. Photo: Gareth Evans
14. SUB: Josh Earl
Josh Earl has experienced a mixed start to life in Tangerine. Photo: Gareth Evans
15. SUB: Finley Munroe
Finley Munroe was introduced off the bench at the weekend. Photo: Gareth Evans
16. SUB: Theo Upton
Theo Upton is eligible to feature in the EFL Trophy as part of the loan agreement with Fylde. Photo: Gareth Evans
17. SUB: Oli Hunter
Youngster Oli Hunter has been a regular on the Blackpool bench so far this season. Photo: Gareth Evans
18. SUB: Shay Mannix
Young forward Shay Mannix could get a first-team opportunity. Photo: Gareth Evans