Both the Seasiders and their Wirral opponents have already qualified, with the result of the upcoming contest only set to decide who finishes first and second.
Blackpool are currently one point better off than Rovers, so anything apart from a defeat would secure them a home game in the next round.
On the back of Saturday’s 3-1 victory over Cardiff City, Ian Evatt is set to rotate his side.
Here’s our predicted XI and bench for the game at Prenton Park:
1. Franco Ravizzoli
With Bailey Peacock-Farrell on international duty with Northern Ireland, Franco Ravizzoli will get his chance in goal. Photo: Gareth Evans
2. Andy Lyons
Andy Lyons could return to the back three after featuring off the bench on Saturday afternoon. Photo: Gareth Evans
3. Olly Casey
Olly Casey could be one of the players asked to go again after Saturday's game. Photo: Gareth Evans
4. George Elder
George Elder featured in the Seasiders' last EFL Trophy outing. Photo: Gareth Evans
5. Scott Banks
Scott Banks could come back into the Seasiders' starting XI at right wing-back. Photo: Gareth Evans
6. Lee Evans
Lee Evans missed Saturday's game through suspension so could be thrown back in for the trip to the Wirral. Photo: Gareth Evans