Both the Seasiders and their Wirral opponents have already qualified, with the result of the upcoming contest only set to decide who finishes first and second.
Blackpool are currently one point better off than Rovers, so anything apart from a defeat would secure them a home game in the next round.
On the back of Saturday’s 3-1 victory over Cardiff City, Ian Evatt is set to rotate his side.
Here’s our predicted XI and bench for the game at Prenton Park:
7. Theo Upton
Theo Upton impressed in the Seasiders' victory over Nottingham Forest U21s last month. Photo: Gareth Evans
8. Emil Hansson
Emil Hansson could start in the more advanced midfield three role. Photo: Gareth Evans
9. Zac Ashworth
Zac Ashworth could feature at left wing-back with Hayden Coulson forced off with an injury against Cardiff. Photo: Gareth Evans
10. Terry Bondo
Terry Bondo will more than likely get an opportunity to start up front. Photo: Gareth Evans
11. Ashley Fletcher
Due to a shortage up front, Ashley Fletcher may be called into action to start again if Evatt wants a senior figure up front to accompany Bondo. Photo: Gareth Evans
12. SUB: Harvey Bardsley
Harvey Bardsley could be the Seasiders back-up goalkeeper option with Peacock-Farrell away with Northern Ireland. Photo: Gareth Evans