At least eight first-team members will be absent, seven through injury and Jordan Thorniley with concussion.
With that in mind, here’s how we think the Seasiders might line up at the DW Stadium...
1. GK - Chris Maxwell
There's certainly a case to be made that Dan Grimshaw deserves his place back in the side, but will Appleton make that change before the World Cup break? Photo: CameraSport - Dave Howarth
2. RB - Callum Connolly
Connolly will slot back in at right-back after serving his one-match ban, freeing up Grant Ward after a difficult night in midweek. Photo: CameraSport - Andrew Kearns
3. CB - Marvin Ekpiteta
The skipper will need to rediscover some form after a well below-par display against Middlesbrough. Photo: CameraSport - Andrew Kearns
4. CB - Rhys Williams
With James Husband injured and Jordan Thorniley likely to miss out again through concussion protocol, Williams will have to be drafted in. Photo: CameraSport - Dave Howarth
5. LB - Dom Thompson
Thompson is likely to get the nod again at left-back despite a poor midweek showing, with Luke Garbutt remaining on the bench. Photo: CameraSport - Rich Linley
6. CM - Kenny Dougall
The Aussie is a certainty to start in midfield given Blackpool's lack of options in the middle of the park. Photo: CameraSport - Dave Howarth