Service 1, which runs every 30 minutes between Fleetwood' s Affinity Outlet and Starr Gate via the Promenade, will not run on Saturday due to driver shortages.
A spokesman for Blackpool Transport said: "Due to the staff shortages we are facing, we regret to inform you that we will not be able to operate a service 1 on Saturday 25.
"We apologise for the inconvenience caused and thank you for your understanding during these difficult times. We will continue to update."
It comes after transport bosses announced timetable cuts last week due to a shortage of drivers.
"This is because the logistics and transport industry across the UK is experiencing a shortage of drivers and has driver vacancy rates of up to 20 per cent," bosses said.
Service cuts came into effect last weekend.
The routes affected by the timetable changes are services 1, 5, 6, 7, 9, 11 and 14.
Other services will not change and trams will continue to run to their current timetable.
On Friday, Blackpool Transport said its number 7 service was delayed in Bispham "due to extensive queuing around the petrol station in Bispham Village," as residents rushed to stock up on fuel amid shortage fears.