Lancashire and Essex both failed to reach the Yorkshire Bank 40 semi-finals after the visitors won a high-scoring contest by 70 runs at Emirates Old Trafford.
A superb 130 for England limited overs batsman Ravi Bopara underpinned the Eagles’ imposing 297 for four, which the Lightning responded to with 227 all out inside 37 overs.
Tom Smith hit an excellent 84-ball 97 but the home side’s task was too stiff.
Tim Phillips nipped in with five for 42 from 7.4 overs as Lancashire lost their last eight wickets for 41 runs, slipping from 186 for two.
Bopara warmed up for England’s two-match Twenty20 series against Australia later this week with his highest List A county score since June 2008, including nine fours and three sixes in 102 balls.